Journal: Cells

Article Title: Phenotypical and Functional Alteration of γδ T Lymphocytes in COVID-19 Patients: Reversal by Statins

doi: 10.3390/cells11213449

Figure Lengend Snippet: Phenotypic analysis of circulating Vδ1+ and Vδ2+ T lymphocytes in hospitalized and recovered COVID-19 patients and in healthy subjects. Distribution of ex vivo memory subsets of Vδ1+ ( A ) and Vδ2+ ( C ) T lymphocytes based on the expression of CD27 and CD45RA in hospitalized and recovered COVID-19 patients, compared to healthy donors. Median is shown. Healthy donors were represented as triangle, COVID-19 patients as circle and COVID-19 recovered as rhombus. Representative dot-plots showing Vδ1+ ( B ) and Vδ2+ ( D ) T lymphocyte memory subset distribution in hospitalized, recovered, and healthy subjects. * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: To assess the ex vivo frequency, phenotype, and exhaustion-marker expression of γδ T lymphocytes, PBMCs were stained with specific fluorochrome-conjugated monoclonal antibodies: APC-Cy7-conjugated anti-human CD45 (clone REA747), PerCP-Vio700-conjugated anti-human CD3 (clone REA613), PE-conjugate anti-human Vδ1 (clone REA173), PE-Vio770-conjugate anti-human Vδ2 (clone REA771), FITC-conjugate anti-human TIM3 (clone F38-2E2), APC-conjugate anti-human PD-1 (clone PD1.3.1.3), PE-Vio615-conjugate anti-human CD27 (clone REA499), and VioBlue-conjugated anti-human CD45RA (clone REA1047) (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Ex Vivo, Expressing

Journal: Cells

Article Title: Impact of Mycobacterium tuberculosis Infection on Human B Cell Compartment and Antibody Responses

doi: 10.3390/cells11182906

Figure Lengend Snippet: Phenotypic distribution frequency of Naive ( A ), Memory ( B ), Switched memory ( C ), and Atypic ( D ) B cells based on the expression of CD19, CD27, and IgD among the groups. We tested 12 LTBI, 17 Active TB, and 16 HD, as shown in the figure. The histograms represent the mean and SE of each group. Significance of differences between groups was compared using the Kruskal–Wallis test, * p < 0.05.

Article Snippet: After stimulation, cells were harvested and stained, first with live/dead (L/D) marker (Zombie dye, Biolegend San Diego, CA, USA), then with mAb anti-human CD19 PerCP clone REA675, mAb anti-human CD27 PE-Vio ® 770 clone REA499, and mAb anti-human IgD FITC clone IgD26 (Miltenyi Biotec Bergisch Gladbach Germany).

Techniques: Expressing

Journal: medRxiv

Article Title: HCMV control in allogeneic stem cell transplant recipients - an analysis of humoral and cellular players beyond antigen-specific T cells in the letermovir era

doi: 10.1101/2025.04.14.25325799

Figure Lengend Snippet: Correlations between HCMV-specific IgG and γδ T cells or NK cells were analyzed across all time points (TPs) and at individual time points. N = 40 patients. ( A ) Heatmap summarizing correlations of HCMV-specific IgG levels with numbers of γδ T cells and their subpopulations. ( B-C ) Correlation of HCMV-specific IgG with total Vδ1 + γδ T-cell ( B ) and effector Vδ1 + γδ T-cell (CD45RA + /CD27 − ) numbers ( C ) in all 40 patients across all time points (large panels) and at individual timepoints (small panels). ( D ) Heatmap summarizing correlations of HCMV-specific IgG levels with numbers of NK cells and “memory-like” NK cells. ( E-F ) Correlation of HCMV-specific IgG with total CD56 dim NK cells ( E ) and mature (CD57 + ) “memory-like” (CD159c + ) CD56 dim NK cells ( F ) across all timepoints (large panels), and at individual timepoints (small panels). Data for HCMV controllers (left) and csCMVi patients (right) are displayed separately. All analyses were performed using Spearman’s rank correlation test. *p < 0.05, **p < 0.01, ***p < 0.001. Abbreviations: alloSCT = allogeneic stem cell transplantation; csCMVi = clinically significant CMV infection; HCMV = human cytomegalovirus; Ig = immunoglobulin; TPs = time points.

Article Snippet: Additional antibodies used consisted of IgG FITC, CD27 PE-Vio615 (Miltenyi), IgM BV421, IgD BV605, CD20 BV650, CD21 AF700 (BioLegend), and Fixable Viability Dye eFluor780.

Techniques: Transplantation Assay, Infection

Journal:

Article Title: Expression of the Interleukin-7 Receptor Alpha Chain (CD127) on Virus-Specific CD8 + T Cells Identifies Functionally and Phenotypically Defined Memory T Cells during Acute Resolving Hepatitis B Virus Infection

doi: 10.1128/JVI.80.7.3532-3540.2006

Figure Lengend Snippet: Phenotypic characterization of HBV-specific CD8+ T cells. For each patient, surface staining of HBV-specific CD8+ T cells was performed using an HBV multimer with a strong ex vivo response (Table ​(Table1)1) and antibodies to CD127 (A), CD38 (B), CCR7 (C), or CD27 (D). The phenotype of HBV-specific CD8+ T cells was analyzed in the early phase (gray bars) and after resolved infection (black bars). Data are shown as the percentages of all multimer-positive CD8+ T cells. Representative density plots of each surface staining from patients 1 and 3 during acute (early) and after resolved infection (late) gated on CD8+multimer-positive cells are shown on the right.

Article Snippet: Anti-CD127 antibody was purchased from Immunotech (Marseille, France), anti-CCR7-PE antibodies were purchased from R&D Systems (Minneapolis, MN), and anti-CD27-PE antibody was purchased from Hoelzel Diagnostika (Cologne, Germany).

Techniques: Staining, Ex Vivo, Infection