Journal:

Article Title: Expression of the Interleukin-7 Receptor Alpha Chain (CD127) on Virus-Specific CD8 + T Cells Identifies Functionally and Phenotypically Defined Memory T Cells during Acute Resolving Hepatitis B Virus Infection

doi: 10.1128/JVI.80.7.3532-3540.2006

Figure Lengend Snippet: Phenotypic characterization of HBV-specific CD8+ T cells. For each patient, surface staining of HBV-specific CD8+ T cells was performed using an HBV multimer with a strong ex vivo response (Table ​(Table1)1) and antibodies to CD127 (A), CD38 (B), CCR7 (C), or CD27 (D). The phenotype of HBV-specific CD8+ T cells was analyzed in the early phase (gray bars) and after resolved infection (black bars). Data are shown as the percentages of all multimer-positive CD8+ T cells. Representative density plots of each surface staining from patients 1 and 3 during acute (early) and after resolved infection (late) gated on CD8+multimer-positive cells are shown on the right.

Article Snippet: Anti-CD127 antibody was purchased from Immunotech (Marseille, France), anti-CCR7-PE antibodies were purchased from R&D Systems (Minneapolis, MN), and anti-CD27-PE antibody was purchased from Hoelzel Diagnostika (Cologne, Germany).

Techniques: Staining, Ex Vivo, Infection